Which 96‑well U‑bottom suspension plates does PromoCell recommend for chondrogenic differentiation of hMSC?
For chondrogenic differentiation of human mesenchymal stem cells (hMSC), it is essential that the cells do not adhere to the culture surface.
During differentiation, hMSCs aggregate and form spheroids that float freely in the medium. Therefore:
During differentiation, hMSCs aggregate and form spheroids that float freely in the medium. Therefore:
- No specific plate brand is required
- Any 96‑well U‑bottom plate that is suitable for suspension culture can be used
- The critical requirement is the U‑bottom geometry, which supports spheroid formation and prevents cell attachment
Products relevant to this topic
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
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Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
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Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
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Mesenchymal Stem Cell Chondrogenic Differentiation Medium
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Serum-free cell culture medium for differentiation of mesenchymal cells into chondrogenic lineages.