Technical library

It has been shown that phenol red has estrogenic properties. Phenol red-free media are therefore generally used in studies evaluating steroid hormone action in cultured, estrogen-responsive cells (Berthois et al., 1986). Furthermore, phenol red can also interfere with some analytical methods like photometric analyses.

Further Information

Berthois et al.; Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500

The formulation of our basal media is proprietary information. If you need to know the concentration of a particular component for your experiments, please contact our Scientific Support Team.

The qualitative and quantitative composition of the supplements can be found on our website and in the data sheets of the specialized media. When there is no such information specified, the composition of the supplements is confidential.

If antibiotics are deemed necessary the recommended final concentrations are: 100 U/ml penicillin + 100 µg/ml streptomycin or 50 µg/ml gentamicin + 50 ng/ml amphotericin B

Please note: Addition of antibiotics can reduce the growth rate of the cells.

Our kidney cells are characterized by their epithelial cell morphology and by cytokeratin expression using a pan-cytokeratin antibody.

Our HREpC (C-12665) comprise a heterogeneous mixture of renal epithelial cells isolated from cortex and medulla. The HRCEpC (C-12660) are derived from renal cortex only.

For the M2 Macrophage Generation Medium, it is extremely important that the shelf life of 2 weeks (after addition of the cytokines) is not exceeded; the yield will quickly decrease thereafter. It is best to use the M2 medium as fresh as possible to avoid discrepancies between M1 and M2 yield.

Most of our subcutaneous preadipocyte lots achieve > 80-90% differentiation when differentiation is induced at P2 (directly after thawing). We generally recommend using cells for differentiation tests that haven't undergone more than 4-5 doublings (a maximum of 1 passage after thawing), as the differentiation ratio will decline with the age of the cells.

Spinning the cells for 15 min at 350 x g has been proven and tested by PromoCell Research & Development. The QC department uses these settings during testing. Any lower centrifugation value (g-force and/or time) will lead to significant cell loss by means of non-sedimented, but intact, macrophages. 

The cells sink down to the bottom of the culture vessel but don't really attach. They retain a roundish morphology and can be rinsed off with culture medium easily.