3D Cell Culture

Delivering physiologically relevant research data relies on models as close to the target biological system as possible. 3D cellular models allow a better understanding of complex biology in a physiologically relevant context. At PromoCell, we have both the expertise and the appropriate systems to support your journey towards the future of in vitro research.

Working with 3D cell culture better mimics the in vivo environment than classical 2D culture (Cukierman E., et al. 2001). Biological processes typically involve multiple cell types and due to their architectural similarity to in vivo tissues, 3D culture can recreate some of this complexity in vitro (Pampaloni F., et al. 2007).

 

3D cell culture refers to the culture of living cells in three dimensions, mimicking tissue and organ-specific microarchitecture (Huh D., et al. 2011). 3D cell growth allows better intercellular contact and signaling (Abbott A. 2003) while allowing cells to differentiate into more complex structures (Cukierman E., et al. 2002).

 

There are various methods for 3D cell culture, each with its own advantages and limitations. Since the choice of a method appropriate for a specific cell-based assay is key for success, in this article we will review four different approaches to 3D cell culture, namely, spheroids, organoids, air-liquid interface, and magnetic 3D culture.

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