From clumps to consistency: Advancing reproducible 3D cancer models

Reliable 3D cancer models are essential for studying tumor biology and therapeutic response. Yet conventional tumorsphere workflows often suffer from inconsistent sphere formation and heterogeneous clumps.

In an upcoming webinar, we’ll introduce a streamlined, matrix free workflow built around our 3D Tumorsphere Medium XF. This approach enables the generation of uniform, robust tumorspheres across multiple cancer types—without additional tools or extracellular matrices. We’ll share data showing improved tumorsphere formation efficiency and long term expansion of patient derived tumor cells while preserving key tumor characteristics.

We’ll then explore how these robust spheroids can be integrated into the MIVO® cancer on chip platform to move from static cultures to dynamic, physiologically relevant systems. 
By recreating key biophysical cues of the tumor microenvironment, this approach supports more predictive studies of tumor behavior and drug response.

What you’ll learn:

• How to generate uniform, reproducible tumorspheres using a matrix free workflow
• Strategies to support cancer stem cell–driven growth across cancer types
• How to integrate 3D spheroids into dynamic cancer on chip systems
• How fluid flow and shear stress improve modeling of tumor invasion and drug efficacy

Join our webinar:
From clumps to consistency: Advancing reproducible 3D cancer models into dynamic cancer-on-chip systems
Thursday, July 16th, 3pm CET / 9AM EST

This webinar is free and open to the public.
Can’t attend live? No problem. We’ll make a recording of the event available on our website and share it via email with those registered.

Alexander Trampe, Ph.D
Cancer Specialist & Project Manager at PromoCell

Alexander Trampe is Project Manager of PromoCell’s Cancer Media Toolbox with nearly 18 years of experience supporting researchers. His expertise centers on cancer cell culture including tumorspheres and more advanced 3D cancer models. He holds a Ph.D. in cell biology from the University of Lübeck and a degree in biochemistry from the University of Bielefeld.

Silvia Scaglione
Director of research at the National Council of Research (CNR), Co- founder & chief scientist of React4Life

As a bioengineering and tissue engineering expert, she has authored over 80 peer-reviewed publications and holds several patents in fluidic technologies and 3D cultures. Her research focuses on developing advanced organ-on-chip platforms, such as the MIVO® system, to accelerate translational research and drug testing. Under her leadership, React4Life has received prestigious international recognitions, including the CPHI Award 2023 and the EU Innovation Radar Award.