How should Accutase be used for cell detachment?
Accutase can be used directly, without dilution, following this short protocol:
- Wash the cells with sterile PBS (without Ca²⁺/Mg²⁺) or Hepes‑BSS
- Add Accutase to the culture vessel (example: 2-3 ml per 25 cm² or 8 ml per 75 cm² TC flask)
- Incubate for 5-20 min at room temperature, or 37°C for faster detachment
- Once the majority of cells has detached, collect the cell suspension, dilute it with fresh medium
- Centrifuge and resuspend the cell pellet in fresh culture medium
Products relevant to this topic
Accutase-Solution
Cat No
C-41310
Accutase-Solution, primary human cell culture tested.
Dulbeccos PBS, w/o Ca++ / Mg++
Cat No
C-40232
Dulbecco's PBS, ready-to-use with or without Ca²⁺ / Mg²⁺
HEPES Buffered Saline Solution, 30 ml
Cat No
C-40000
HEPES Buffered Saline Solution (30 mM) available in different sizes.
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
Cat No
C-12971
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
Cat No
C-12974
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
Cat No
C-12977
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Pericytes from Placenta (hPC-PL)
Cat No
C-12980
Human Pericytes isolated from human placenta tissue.