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According to our experience; they can be maintained for 3 to a maximum of 7 days. However; the morphology will change and they will look more and more "degenerate".
Some of our customers have successfully used PromoCell Endothelial Cell Growth Medium MV2 (C-22022) when co-culturing Human Coronary Artery Endothelial Cells (HCAEC; C-12221) and Normal Human Dermal Fibroblasts (NHDF; C-12300).
- Right coronary artery
- Left main coronary artery
- Circumflex coronary artery and
- Left anterior descending coronary artery.
- FBS (or BSA) can be substituted for HSA; however we do not recommend this as the FBS will lead to unpreferable immunologic stimulation of human macrophages.
- The addition of EDTA to the PBS is to further augment the “anti-clumping” activity of the Ca2+/Mg2+ free PBS.
Usually we do not recommend specific plasticware but we have found that with PromoCell’s Dendritic Cell and Macrophage Generation Media choice of plasticware can have a lot of an influence. For our Dendritic Cell Generation Media we recommend to use tissue culture vessels from BD FalconTM.
Both cytokines are produced in E.coli.