JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Home
RESOURCES
Technical Library

Filters

Keratinocytes (1)

Skeletal Muscle (1)

3D Skin Models (1)

Technical Library

Sort by: Bestseller

Bestseller

Name (A-Z)

Name (Z-A)

According to our experience; they can be maintained for 3 to a maximum of 7 days. However; the morphology will change and they will look more and more "degenerate".

The Vena saphena section that we use for HSaVEC isolation originates from the thigh.

All our Human Uterine Fibroblasts (HUF) are prepared from the myometrium.

Some of our customers have successfully used PromoCell Endothelial Cell Growth Medium MV2 (C-22022) when co-culturing Human Coronary Artery Endothelial Cells (HCAEC; C-12221) and Normal Human Dermal Fibroblasts (NHDF; C-12300).

Our HCASMC (C-12221) are isolated from the large arteries; i.e. from

Right coronary artery
Left main coronary artery
Circumflex coronary artery and
Left anterior descending coronary artery.

Yes primary mouse keratinocytes grow well in this media when you reduce the CaCl₂ concentration to 0.025 mM - 0.05 mM CaCl₂ (optimal Ca concentration for primary human keratinocytes is 0.06 mM).

FBS (or BSA) can be substituted for HSA; however we do not recommend this as the FBS will lead to unpreferable immunologic stimulation of human macrophages.

The addition of EDTA to the PBS is to further augment the “anti-clumping” activity of the Ca²⁺/Mg²⁺ free PBS.

Usually we do not recommend specific plasticware but we have found that with PromoCell’s Dendritic Cell and Macrophage Generation Media choice of plasticware can have a lot of an influence. For our Dendritic Cell Generation Media we recommend to use tissue culture vessels from BD Falcon^TM.

Usually; we do not recommend specific plasticware but we have found that with PromoCell’s Dendritic Cell and Macrophage Generation Media; choice of plasticware can have a lot of an influence. For our M1-/M2 Macrophage Generation Media XF we recommend the Nunc plasticware with Nunclon surface - as not only will the detachment efficiency vary (up to 20%); but also the efficiency of the differentiation process itself may be altered.

Samples in RNAlater will NOT freeze at -20°C they are liquid at 4°C and -20°C. The samples can be stored at 4°C for up to 1 month and are stable at room temperature for up to one week. At -20°C they can be stored indefinitely.

Our customers have successfully used TC flasks and dishes from all the leading cell culture plastic suppliers to grow PromoCell's primary human cells. We do not have any knowledge whether the dishes from local TC plastic suppliers work in the same way. We recommend to first test whether these brands provide the same good performance as the plastic of the leading manufacturers.

Both cytokines are produced in E.coli.

Choose your region

North America

Europe

Asia

Technical Library