Air-Liquid Interface Medium

Serum-free and BPE-free cell culture medium for an optimal and standardized culture of human bronchial epithelial cells at the air-liquid interface (ALI).

Air-Liquid Interface Medium
C-21080
500 ml
$ 251.00

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Air-Liquid Interface Medium

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Our Air-Liquid Interface Medium (ALI-Airway) is a serum- and bovine pituitary extract (BPE)-free cell culture medium for the standardized generation of a 3D airway model with a reproducible epithelial barrier function using Human Bronchial Epithelial Cells (HBEpC). The medium contains a low level of growth factors to maintain a non-proliferating cell population and it suits perfect for long-term culture on a semipermeable membrane. The functionality of the airway epithelial cells can be monitored by a fast development of an epithelial barrier formed by tight junctions.

In addition to the ALI-Airway medium, we provide HBEpCs pre-screened for epithelial barrier formation in our ALI-Airway medium. When culturing ALI pre-screened HBEpC in our ALI-Airway Medium, we guarantee a stable transepithelial electrical resistance (TEER) of > 500 Ω*cm2 until 4 weeks of culture at air-liquid-interface. Together with our Airway Epithelial Cell Growth Medium and ALI pre-screened HBEpC, the ALI-Airway medium constitute a standardized system to facilitate reproducible and physiologically relevant respiratory research.

Advantages:

  • Minimize lot-to-lot variation by optimized BPE-free and serum-free formulation
  • Stable and uniform TEER values of > 500 Ω*cm2 until 4 weeks of culture
  • ALI pre-screened HBEpC available on request , optionally from HLA-typed and COPD/Asthma donors
  • Differentiated bronchial epithelial cells will be kept in a healthy status with a cell viability > 70 %

Components:

  • 1x Basal Medium, 500ml
  • 1x SupplementMix for 500ml

Adding the SupplementMix to the Basal Medium results in complete Growth Medium.

 

Figure 1. TEER values of barrier forming HBEpC from two differen ALI pre-screened donors (Donor 1 and Donor 2 PromoCell ALI Medium) in comparison to a competitors ALI medium over a culture period of 28 days post-airlift.
Figure 1. TEER values of barrier forming HBEpC from two differen ALI pre-screened donors (Donor 1 and Donor 2 PromoCell ALI Medium) in comparison to a competitors ALI medium over a culture period of 28 days post-airlift.
Figure 2. ALI pre-screened HBEpC show (A) a typical morphology and (B) high viability in ALI-Airway Medium after 28 days post-airlift. At this time TEER measurements should be >500 Ω*cm2 indicating a monolayer with high integrity and barrier function.
Figure 2. ALI pre-screened HBEpC show (A) a typical morphology and (B) high viability in ALI-Airway Medium after 28 days post-airlift. At this time TEER measurements should be >500 Ω*cm2 indicating a monolayer with high integrity and barrier function.

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