Air-Liquid Interface Medium

Serum-free and BPE-free cell culture medium for an optimal and standardized culture of human bronchial epithelial cells at the air-liquid interface (ALI).

Air-Liquid Interface Medium
C-21080
500 ml
$ 251.00

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Air-Liquid Interface Medium

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Our Air-Liquid Interface Medium (ALI-Airway) is a serum- and bovine pituitary extract (BPE)-free cell culture medium for the standardized generation of a 3D airway model with a reproducible epithelial barrier function using Human Bronchial Epithelial Cells (HBEpC). The medium contains a low level of growth factors to maintain a non-proliferating cell population and it suits perfect for long-term culture on a semipermeable membrane. The functionality of the airway epithelial cells can be monitored by a fast development of an epithelial barrier formed by tight junctions.

In addition to the ALI-Airway medium, we provide HBEpCs pre-screened for epithelial barrier formation in our ALI-Airway medium. When culturing ALI pre-screened HBEpC in our ALI-Airway Medium, we guarantee a stable transepithelial electrical resistance (TEER) of > 500 Ω*cm2 until 4 weeks of culture at air-liquid-interface. Together with our Airway Epithelial Cell Growth Medium and ALI pre-screened HBEpC, the ALI-Airway medium constitute a standardized system to facilitate reproducible and physiologically relevant respiratory research.

Advantages:

  • Minimize lot-to-lot variation by optimized BPE-free and serum-free formulation
  • Stable and uniform TEER values of > 500 Ω*cm2 until 4 weeks of culture
  • ALI pre-screened HBEpC available on request , optionally from HLA-typed and COPD/Asthma donors
  • Differentiated bronchial epithelial cells will be kept in a healthy status with a cell viability > 70 %

Components:

  • 1x Basal Medium, 500ml
  • 1x SupplementMix for 500ml

Adding the SupplementMix to the Basal Medium results in complete Growth Medium.

 

Epithelial barrier integrity graph
Figure 1. TEER values of barrier forming HBEpC from two differen ALI pre-screened donors (Donor 1 and Donor 2 PromoCell ALI Medium) in comparison to a competitors ALI medium over a culture period of 28 days post-airlift.
Epithelial barrier integrity graph
Figure 1. TEER values of barrier forming HBEpC from two differen ALI pre-screened donors (Donor 1 and Donor 2 PromoCell ALI Medium) in comparison to a competitors ALI medium over a culture period of 28 days post-airlift.
Figure 2. ALI pre-screened HBEpC show (A) a typical morphology and (B) high viability in ALI-Airway Medium after 28 days post-airlift. At this time TEER measurements should be >500 Ω*cm2 indicating a monolayer with high integrity and barrier function.
Figure 2. ALI pre-screened HBEpC show (A) a typical morphology and (B) high viability in ALI-Airway Medium after 28 days post-airlift. At this time TEER measurements should be >500 Ω*cm2 indicating a monolayer with high integrity and barrier function.

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