Is PCCS the best medium for isolating cancer stem cells from fresh tumor tissue?

Yes. The Primary Cancer Culture System (PCCS, Cat. No. C‑28081) is highly suitable for the isolation of cancer stem cells (CSCs) from fresh tumor tissue.
PCCS is designed to be highly selective for cancer stem cells. Under these culture conditions:

• Cancer stem cells are maintained and enriched
• Differentiated tumor cells and non‑malignant cells are eliminated within a short time
• The resulting culture is therefore strongly enriched for CSC‑like cells

This selectivity makes PCCS particularly well suited for establishing patient‑derived cancer stem cell cultures.
For additional details and experimental background, please refer to the Application Note: "Isolation of patient-derived primary cancer cells".