Cancer cells, whether within tumors or established cancer cell lines, are heterogeneous populations that can include:
Tumor-initiating, slow-growing cancer stem cells
Fast-growing aggressive cancer cells
Tumor-associated cancer cells
Immune cells and other cells (depending on your experimental set-up)
To help you isolate, cultivate for the long-term, and co-culture your desired cell types, we’ve created a toolbox to do just that. Depending on your application, there’s a toolbox to enrich, eliminate, or co-culture specific cancer cell types.
All three media options in the toolbox will maintain cancer stem cells (CSCs). This focus on CSCs, despite them making up 1–2% of the cell population, is because they play a critical role in tumor initiation, progression, and metastasis. It’s therefore essential to maintain CSCs whichever tumor or cancer cell lines you work with. All three media types are synthetic, animal component-free, and xeno-free, but rich enough to allow growth and long-term cultivation in either 2D or 3D. You can use these media for most established cell lines and the establishment of cell lines from different cancer types. This flexibility of the toolbox allows you to use it across a variety of applications, such as cancer stem cell research, establishing standardized cell lines from biopsies for drug discovery, or co-culturing cancer and immune cells for cell-based therapy development.
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3D Tumorsphere Medium XF
Serum-free and xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
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3D Tumosphere Medium XF
Serum-free and xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
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Cancer Cell Line Medium XF
Serum-free and xeno-free medium for the standardized in vitro cultivation of established cancer cell lines. Fibronectin or vitronectin coating required.
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NCCD Reagent
NCCD Reagent is part of our Primary Cancer Culture System and is crucial for the pre-treatment of the culture vessel.
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Primary Cancer Culture System
A complete defined and animal-component free cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts.
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Freezing Medium Cryo-SFM Plus
Defined, animal-component free, and protein-free cryopreservation medium for optimal cell storage.
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