Cell pellet consisting of 1 million cells dissolved in 200 µl RNAlater® for subsequent RNA, DNA or protein analysis.
Note: Cell pellets cannot be revived.
Our primary Human Small Airway Epithelial Cells (HSAEpC) are obtained from the distal portion of the human respiratory tract, specifically the 1 mm bronchiole area. These cells, positive for cytokeratin, are ideal for functional studies, particularly in investigating respiratory disorders like asthma and pulmonary inflammation.
Samples in RNAlater will NOT freeze at -20°C they are liquid at 4°C and -20°C. The samples can be stored at 4°C for up to 1 month and are stable at room temperature for up to one week. At -20°C they can be stored indefinitely.
PromoCell cell pellets are made from > 1 million cells and are dissolved in 200 µl RNAlater.
The majority of PromoCell cell pellets (C-14**) are prepared 1 passage after thawing the cryopreserved cells.
Examples:
HUVEC and HUAEC correspond to P1 after thawing; therefore the pellets are frozen in P2.
SMC or keratinocytes or epithelial cells correspond to P2 after thawing; therefore the pellets are frozen in P3.
In contrast; our blood cells are cryopreserved directly after cell isolation. These pellets are prepared after thawing the cryovials with no further cultivation step.
Yes RNAlater Solution will denature proteins. Therefore protein obtained from PromoCell cell pellets will be suitable for applications such as Western blotting or 2D gel electrophoresis but not for applications that require native protein.
There are two options for isolating RNA from cells stored in RNAlater Solution:
1) The solution is removed from the cells prior to extraction by centrifugation at 5;000 x g for 10 minutes at 4°C.
Note: Because of the density of RNAlater® solution; greater centrifugal forces are required to spin down the cells.
2) If no pellet is visible after centrifugation; RNA can also be purified directly from the RNAlater® solution. This can be done by adding 2 ml of 10x lysis buffer; and proceeding normally.
PromoCell cell pellets (C-14**) are an easily accessible source of DNA RNA and proteins.
No the PromoCell cell pellets (C-14**) are frozen at -20°C and cannot be revived. Their main application is to analyze RNA or protein. Cryopreserved cells that can be revived are available from the same donors. Please contact our Technical Customer Support if you need matched viable cells.
PromoCell cell pellets (C-14**) can be stored indefinitely at -20°C and are stable for up to 1 month at 4°C and up to one week at room temperature.
Please note: The samples do not freeze at -20°C.
Tools for respiratory research
Thawing frozen cells – detailed protocol for human primary cells
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