Can PromoCell M1 macrophages be cultured after thawing in MEM α containing 10% FBS and GM‑CSF?
No. PromoCell has not tested the cultivation of assay‑ready M1 macrophages in MEM α supplemented with FBS.
Because PromoCell’s M1 macrophage system is fundamentally different from MEM‑based media, we cannot predict whether cell viability, phenotype, or function would be maintained under these conditions.
For reliable performance, we strongly recommend using PromoCell M1 Macrophage Generation Medium XF in combination with fibronectin‑coated culture vessels, as this is the only condition validated by PromoCell.
Because PromoCell’s M1 macrophage system is fundamentally different from MEM‑based media, we cannot predict whether cell viability, phenotype, or function would be maintained under these conditions.
For reliable performance, we strongly recommend using PromoCell M1 Macrophage Generation Medium XF in combination with fibronectin‑coated culture vessels, as this is the only condition validated by PromoCell.
Products relevant to this topic
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12914
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M1-Macrophage Generation Medium XF (incl. GM-CSF).
M1-Macrophage Generation Medium XF
Cat No
C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.