Powered by BiozKeratinocyte Growth Medium 2
Serum-free cell culture medium for keratinocytes from the epidermis.
Our Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells.
Although all our media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for horse and porcine keratinocytes, as well as mouse and rat keratinocytes after adapting the CaCl₂ concentration. In addition, it is suited for rat corneal keratinocytes.
View a list of references where this medium was used with other species/cell types.
Components:
- Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- Growth Medium Kit: Includes Basal Medium and SupplementPack
- SupplementMix: Contains all media supplements premixed in one vial
- SupplementPack: Contains all media supplements as individual vials
| Media supplement | Final supplement concentration |
| Bovine Pituitary Extract | 0.004 ml/ml |
| Epidermal Growth Factor (recombinant human) | 0.125 ng/ml |
| Insulin (recombinant human) | 5 μg/ml |
| Hydrocortisone | 0.33 μg/ml |
| Epinephrine | 0.39 μg/ml |
| Transferrin (recombinant human) | 10 μg/ml |
| CaCl₂ | 0.06 mM |
Please note: Due to production reasons, we have changed the label on the basal medium bottle of our Ready-to-use growth media from “Growth Medium” to “Basal Medium.” This change only affects the label; the volume and formulation remain the same. Please note that the catalogue number on the basal medium bottle differs from that of the complete Ready-to-use growth medium.
Recommended products
Normal Human Epidermal Keratinocytes (NHEK)
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
DetachKit
DetachKit for gentle and effective detachment of adherent primary human cells. Trypsin/EDTA ratio of 0.04%/0.03%.