DetachKit

Our DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells.

The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

Our DetachKit was designed for the safe and efficient detachment of primary human cells in routine subculturing. Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA solution and TNS (Trypsin Neutralizing Solution). The Trypsin/EDTA is a ready-to-use solution standardized to 700 BAEE-U/ml enzymatic activity, ensuring consistent and reliable cell detachment performance. This activity level is functionally equivalent to a 0.04% solution. The formulation is suitable for a broad range of cell types, including primary cells and sensitive cells. The EDTA concentration is 0.03% (w/v).

HEPES BSS contains 30 mM HEPES, D-glucose, NaCl, KCl, sodium phosphate and phenol red. TNS contains 0.05% trypsin inhibitor from soybean and 0.1% bovine serum albumin (BSA).