Which medium does PromoCell recommend for endothelial cell transfection?

Successful transfection of endothelial cells depends on several factors, including cell viability and density, the transfection reagent, the type and quality of the nucleic acid (e.g. plasmids, siRNA, oligonucleotides), and the culture medium and supplements used.

Recommended medium for transfection
PromoCell recommends using heparin‑free culture conditions during the transfection step, as heparin can reduce transfection efficiency.

• Preferred option: Endothelial Cell Growth Medium MV2 (C‑22022 / C‑22121), which is heparin‑free
• Alternative option: Use one of the following Growth Medium Kits without adding the ECGS/heparin supplement to the basal medium:
  • Endothelial Cell Growth Medium Kit (C‑22110)
  • Endothelial Cell Growth Medium 2 Kit (C‑22111)
  • Endothelial Cell Growth Medium MV Kit (C‑22120)

Important note
Before and after transfection, endothelial cells should be cultured in complete Growth Medium including heparin to ensure optimal cell growth and recovery.