When do I need PromoCell’s Monocyte Attachment Medium (C‑28051)?

PromoCell Monocyte Attachment Medium (C‑28051) is used when working with freshly isolated peripheral blood mononuclear cells (MNC) and a rapid, efficient adherence‑based selection of monocytes is required.
 
Purpose
The Monocyte Attachment Medium enables:

• Efficient adherence of monocytes within ~1 hour
• Selective enrichment of the monocyte fraction
• Preservation of optimal cell health

Key advantage
When using Monocyte Attachment Medium, time‑consuming and costly immunomagnetic monocyte purification steps are not required. The medium allows direct progression to downstream applications, such as macrophage or dendritic cell generation, without prior monocyte isolation.
 
Further information:
Detailed protocols for macrophage and dendritic cell generation from freshly isolated monocytes are provided in the corresponding PromoCell Application Notes.

• Generation of monocyte derived Dendritic Cells (moDCs)
• Differentiation of M1- or M2-Macrophages from PBMC/Monocytes