How should I handle PromoCell proliferating cells after arrival?

Please follow the instructions below when handling PromoCell proliferating cells upon arrival.

Initial handling after delivery
1. Unpack the shipment immediately and place the T25 flask(s) into a 37°C incubator with the cap closed.
2. Allow the cells to recover for approximately 3 hours.
3. After incubation, check the cell confluency under the microscope.

Medium change
If the cell density is below 70% confluency, aspirate the medium under sterile conditions and add 5-10 ml of the appropriate Growth Medium.

Subculturing
Once the cells have reached more than 70% confluency, they should be subcultured according to the subcultivation protocol provided in the Instruction Manual of the respective cell type.