Has PromoCell tested MSC Adipogenic Differentiation Medium 2 without fibronectin? Is fibronectin coating necessary?
Human mesenchymal stem cells (MSCs) are able to attach without fibronectin coating when cultured in MSC Adipogenic Differentiation Medium 2.
However, under these conditions the risk of cell detachment during adipogenic differentiation is significantly higher.
Recommendation
To ensure stable cell attachment throughout the differentiation process, we strongly recommend using fibronectin‑ or vitronectin‑coated tissue culture vessels.
However, under these conditions the risk of cell detachment during adipogenic differentiation is significantly higher.
Recommendation
To ensure stable cell attachment throughout the differentiation process, we strongly recommend using fibronectin‑ or vitronectin‑coated tissue culture vessels.
Products relevant to this topic
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
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Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
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Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
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Cell culture medium for differentiation of human mesenchymal cells into adipogenic lineages.
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