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Can PromoCell normal human cells be grown in standard media like DMEM or RPMI 1640 supplemented with 10% FBS?

In general, no. PromoCell Normal Human Cells are optimized for use with PromoCell Growth Media, which have specialized and more complex formulations than standard media such as DMEM or RPMI 1640 supplemented with FBS.
Unlike immortalized cell lines, primary human cells have significantly higher and more specific requirements for nutrients, growth factors, and culture conditions. Classical media formulations typically do not support optimal growth or performance of PromoCell primary cells.

Products relevant to this topic

Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
Cat No C-12001
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
708,00 €
Normal Human Epidermal Keratinocytes (NHEK) adult, single donor
Normal Human Epidermal Keratinocytes (NHEK) adult, single donor
Cat No C-12003
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
719,00 €
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled
Cat No C-12005
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
596,00 €
Normal Human Epidermal Keratinocytes (NHEK) adult, pooled
Normal Human Epidermal Keratinocytes (NHEK) adult, pooled
Cat No C-12006
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
582,00 €
Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskin isolated in Growth Medium 3, single donor
Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskin isolated in Growth Medium 3, single donor
Cat No C-12011
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
708,00 €
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, single donor
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, single donor
Cat No C-12013
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
719,00 €
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled
Cat No C-12015
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
596,00 €
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, pooled
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, pooled
Cat No C-12016
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
582,00 €

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