To what extent will CD34⁺ progenitor cells proliferate in Hematopoietic Progenitor Cell Expansion Medium XF (C‑28021)?
For optimal expansion, human CD34⁺ progenitor cells are cultured in PromoCell Hematopoietic Progenitor Cell Expansion Medium XF (C‑28021) supplemented with Cytokine Mix E (C‑39890) or an alternative cytokine cocktail suitable for progenitor cell expansion.
Cytokine Mix E contains the recombinant human cytokines TPO, SCF, Flt‑3 ligand, and IL‑3, which support robust proliferation of CD34⁺ cells.
Under these conditions:
Cytokine Mix E contains the recombinant human cytokines TPO, SCF, Flt‑3 ligand, and IL‑3, which support robust proliferation of CD34⁺ cells.
Under these conditions:
- Strong expansion of CD34⁺ progenitor cells is typically maintained for approximately 2 weeks.
- The expansion factor of CD34⁺ cells is usually in the range of 75‑ to 200‑fold, depending on donor material and culture conditions.
Products relevant to this topic
Hematopoietic Progenitor Cell Expansion Medium XF
Cat No
C-28021
Serum-free and xeno-free medium for expansion of primitive human hematopoietic cells.
373.00 CHF
Cytokine Mix E for HPC-Expansion Medium XF
Cat No
C-39890
Serum-free and xeno-free medium for expansion of primitive human hematopoietic cells.
538.00 CHF