Are there differences in the cultivation protocol for MSCs compared to other cell types?
Yes. Human mesenchymal stem cells (hMSCs) require a few cell‑type‑specific handling adjustments compared to most other primary cell types.
Key differences in MSC cultivation:
Key differences in MSC cultivation:
- Early medium change after seeding: When using MSC Growth Medium XF (C‑28019), we recommend replacing the medium 3–4 hours after seeding. In contrast, for most other cell types and growth media (including MSC Growth Medium 2), the first medium change is typically performed 16–24 hours after seeding.
- Pre‑coating of culture vessels: When using MSC Growth Medium XF, MSC Neurogenic Differentiation Medium, MSC Adipogenic Differentiation Medium 2, or MSC Osteogenic Differentiation Medium, tissue culture vessels must be pre‑coated as described in the Instruction Manual.
- Detachment method: We strongly recommend using Accutase (C‑41310) for detaching MSCs, as it is gentler and helps preserve cell viability. If trypsin is used, the contact time should not exceed 2 minutes.
Products relevant to this topic
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