Are there differences in the cultivation protocol for MSCs compared to other cell types?
Yes. Mesenchymal stem cells (MSCs) require a few cell‑type‑specific handling adjustments compared to most other primary cell types.
Key differences in MSC cultivation:
Key differences in MSC cultivation:
- Early medium change after seeding: When using MSC Growth Medium XF (C‑28019), we recommend replacing the medium 3–4 hours after seeding. In contrast, for most other cell types and growth media (including MSC Growth Medium 2), the first medium change is typically performed 16–24 hours after seeding.
- Pre‑coating of culture vessels: When using MSC Growth Medium XF, MSC Neurogenic Differentiation Medium, MSC Adipogenic Differentiation Medium 2, or MSC Osteogenic Differentiation Medium, tissue culture vessels must be pre‑coated as described in the Instruction Manual.
- Detachment method: We strongly recommend using Accutase (C‑41310) for detaching MSCs, as it is gentler and helps preserve cell viability. If trypsin is used, the contact time should not exceed 2 minutes.
Products relevant to this topic
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
Cat No
C-12974
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
Cat No
C-12977
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Mesenchymal Stem Cell Growth Medium 2
Cat No
C-28009
Low-serum cell growth medium for mesenchymal stems cells.
Mesenchymal Stem Cell Chondrogenic Differentiation Medium
Cat No
C-28012
Serum-free cell culture medium for differentiation of mesenchymal cells into chondrogenic lineages.
Mesenchymal Stem Cell Osteogenic Differentiation Medium
Cat No
C-28013
Cell culture medium for differentiation of mesenchymal cells into osteogenic lineages.
Mesenchymal Stem Cell Neurogenic Differentiation Medium
Cat No
C-28015
Serum-free cell culture medium for differentiation of mesenchymal cells into neurogenic lineages.
Mesenchymal Stem Cell Adipogenic Differentiation Medium 2
Cat No
C-28016
Cell culture medium for differentiation of human mesenchymal cells into adipogenic lineages.
Mesenchymal Stem Cell Growth Medium 2, phenol red-free
Cat No
C-28017