Technical library

Changing the medium every 2-3 days, the neuronally differentiated MSC can be kept in culture for up to 2 weeks.

Human Mesenchymal Stem Cells have retained the ability to differentiate into a variety of cell types including fat cells, chondrocytes, and osteoblasts. PromoCell supply hMSC from 3 different tissues: bone marrow (hMSC-BM; C-12974), umbilical cord matrix (hMSC-UC; C-12971), and adipose tissue (hMSC-AT; C-12977).

• MSC-BM show very good differentiation into bone cells but also into chondrocytes and fat cells when the respective Differentiation Media is used.
• MSC-UC have a high potential to differentiate into chondrocytes but only weak potential for fat or bone cell differentiation.
• In contrast, MSC-AT differentiate very well into fat and bone cells but only moderately into chondrocytes.

In other words, to obtain a high percentage of bone cells, MSC-BM or MSC-AT are the cells of choice. There are of course lot-to-lot variations and the differentiation will decrease from passage to passage. If you need MSCs with a particularly high osteoblast differentiation capacity, you can contact our Scientific Support before placing your order so that we can select an appropriate cell lot for you.

When cultured in Mesenchymal Stem Cell Growth Medium 2 (C‑28009), PromoCell human Mesenchymal Stem Cells (hMSC‑BM, hMSC‑AT, hMSC‑UC) typically show population doubling times of 20- 30 hours. This value represents the mean population doubling time, including the lag phase, measured over 10 population doublings. The Quality Control specification is ≤ 30 hours.

From seeding to subculture: If hMSCs are seeded at a density of 4,000 cells/cm²:

• Cells usually reach subconfluency within 4-7 days
• At this point, they are ready for subculturing

Actual growth rates may vary slightly depending on:

• donor source
• passage number
• handling and culture conditions

Our MNC lots are checked for the rate of lymphocytes, monocytes and granulocytes w/o staining by FSC/SSC using a flow cytometer. A sample plot is attached. For our purified CD14⁺ monocytes we additionally perform a CD14⁺ staining.

It takes 7-8 days to generate fully mature myeloid dendritic cells.

According to our experience, they can be maintained for 3 to a maximum of 7 days. However, the morphology will change and they will look more and more "degenerate".

The Vena saphena section that we use for HSaVEC isolation originates from the thigh.

Juvenile HDMEC (C-12210) are isolated from the dermis of foreskin. Adult HDMEC (C-12212) are isolated from different regions. The localizations include cheek, temple, breast, upper arm, and labia. Please contact our Technical Customer Support if you need HDMEC from a particular part of the body.

Our HPAEC (C-12241) are harvested directly from the pulmonary artery. For their isolation, the vessel is explanted right after the position where the artery leaves the heart, including the bifurcation. HPAEC represent the innermost cell layer (i.e. the endothelial cells) of the pulmonary artery. We also supply pulmonary microvascular endothelial cells (HPMEC; C-12281) isolated from the capillaries of peripheral lung tissue.

For routine in vitro angiogenesis assays, Human Umbilical Vein Endothelial Cells (HUVEC) are the most commonly used endothelial cell type due to their availability, robust growth characteristics, and reliable performance in assays such as tube formation, migration, and sprouting assays.
However, angiogenesis in vivo primarily involves the formation of new capillaries from the microvascular endothelium. Therefore, Human Dermal Microvascular Endothelial Cells (HDMEC) and other primary human microvascular endothelial cells may provide a more physiologically relevant model for studying angiogenesis.
Compared with HUVEC, microvascular endothelial cells more closely represent the small-vessel compartment involved in tissue vascularization and may therefore yield more biologically relevant responses to angiogenic stimuli.
 
Recommendation:

• HUVEC are well suited for routine screening, assay development, and comparative studies.
• HDMEC or other microvascular endothelial cells are recommended when a physiologically relevant model of capillary angiogenesis is required.