Technical library

Upon arrival, the basal media should be stored between 4°C and 8°C, the supplements at -20°C.

Please do not freeze our cell culture media. Freezing can lead to irreversible precipitation of media components and the quality can no longer be assured.

Yes, you can use accutase to detach Normal Human Cells. Accutase acts very gently on the cells. Cell membranes and surface epitopes will not be harmed. It is therefore mostly used for applications that require unchanged surface markers, e.g. for flow cytometry, or for detachment of very sensitive cells.

For best results, we recommend using HEPES BSS to wash cells prior to trypsinization.
PromoCell Trypsin/EDTA (ready‑to‑use) and PromoCell TNS (Trypsin Neutralizing Solution) are both based on HEPES‑buffered Salt Solution (HEPES BSS). Using HEPES BSS for the washing step ensures optimal compatibility with these reagents.
Phosphate‑buffered saline (PBS) can also be used for washing; however, HEPES BSS is normally the preferred option.

We recommend using the PromoCell DetachKit (C‑41200) for subculturing PromoCell normal human cells.
The DetachKit was specifically designed for the safe and efficient detachment of primary human cells during routine subculture and consists of three ready‑to‑use components:

• HEPES BSS (HEPES‑buffered Saline Solution)
• Trypsin/EDTA Solution
• TNS (Trypsin Neutralizing Solution)

Important note
Many PromoCell culture media contain low serum levels or no serum at all. These media are not suitable for inactivating trypsin during subculture.
The Trypsin Neutralizing Solution included in the DetachKit ensures reliable and controlled trypsin inactivation under serum‑free or low‑serum conditions.

Alternative detachment method
As an alternative, PromoCell Accutase Solution may be used for certain cell types.
Please refer to the Manual of the respective cell type for guidance on whether Accutase is suitable and recommended.

The population doubling time (PDT; also called generation time, tg) is typically calculated during the logarithmic (exponential) growth phase of a cell culture. It describes the time required for a cell population to double in number.
 
Formula 
tg = t / n
 
where:
- tg = population doubling time (hours)
- t = time interval (in hours) between two cell counts
- n = number of population doublings during this time interval

Certificates of Analysis (CoA) for PromoCell cells can be downloaded directly from the PromoCell CoA search page.

How to retrieve your CoA

• Go to the PromoCell CoA search page.
• Enter the lot number shown on the cryovial or tissue culture flask.
• Press Enter or click the Search icon to retrieve the corresponding Certificate of Analysis.

 

You need to have experience working under sterile conditions and under a laminar flow hood. It is of advantage to have experience with other cell types and/or cell lines. If you are a beginner in cell culture and would like to establish a cell culture lab, we will assist you in working with PromoCell Normal Human Cells.

Serum turbidity is most commonly caused by cryoprecipitation of lipid components that occurs during freezing and thawing.
The more frequently serum is subjected to freeze–thaw cycles, the more pronounced the turbidity typically becomes. This effect does not necessarily indicate contamination or loss of functionality, but rather physical precipitation.
 
How to minimize serum turbidity
To reduce turbidity, we recommend the following handling practices:

• Aliquot the serum before freezing
• Store aliquots at −20°C
• Thaw only the required aliquot immediately before use
• Avoid repeated freeze–thaw cycles

Differentiation of hMSC into mature adipocytes takes approx. 2 weeks. You can keep the adipocytes for up to 3 weeks in the MSC Adipogenic Differentiation Medium. After 3 weeks we recommend to switch to PromoCell's Adipocyte Nutrition Medium (C-27438).