Airway Epithelial Cell Growth Medium XF (prf)

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Airway Epithelial Cell Growth Medium XF is a xeno-, serum-, and phenol red-free formulation optimized for the in vitro cultivation of epithelial cells from large air passages, including HBEpC, HNEpC, and HTEpC.

Key features

• Optimized for in vitro cultivation of epithelial cells from large air passages (HBEpC, HNEpC, HTEpC)
• Xeno-free and serum-free
• Phenol red-free
• Supplied as a 500 ml bottle of Airway Epithelial Cell Basal Medium XF (prf) with premixed SupplementMix
• Average population doubling time (including lag phase) over 3 passages ≤70 h

Product benefits

• Physiologically relevant growth: Supports cell growth, cobblestone morphology, and airway-specific functions that closely mimic the in vivo environment without the need for small-molecule additives.
• Regulatory compliance: Xeno-free composition ensures batch-to-batch uniformity, reducing variability and improving reproducibility—ideal for large-scale manufacturing.
• Phenol red-free formulation: Eliminates non-functional ingredients for sensitive applications.
• No compromise in cell growth: Achieves equal or greater population doublings compared to BPE-containing and serum-free media.
• Supports primary cell isolation: Proven for isolation of HBEpC, HNEpC, and HTEpC from fresh bronchial, nasal, and tracheal tissues without additional medium components.
• Proven 3D culture performance: Supports the formation of functional airway organoids—rotating spheroid-like structures with predominantly apical-out cilia—for ≥28 days in basal membrane extract.