Antibiotics are routinely used in cell cultures to prevent bacterial infections. But there are side effects: Studies show that they impair cell growth and differentiation. With good laboratory practice, the use of antibiotics is unnecessary.
A cell culture is a world of its own. The culture dish contains nutrients within the media that cells need to thrive and prosper….
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Some human mesenchymal stem cells are more equal than others – origins and differences
It is a scientific success story that started approximately 40 years ago – and was initially met with skepticism. Although the pioneers of mesenchymal stem cell research were initially criticized, today thousands of publications and hundreds of clinical trials focus on multipotent cells. New MSC research is suggesting that in the end,…
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PromoCells in Space – Ready For Lift-Off
Three, Two, One, Zero: Two research groups working in space life sciences are investigating the behavior of cells from PromoCell in microgravity on the International Space Station. The launch of a Falcon 9 rocket coupled to a Dragon spacecraft is planned for December 15th in Florida, USA.
Gravity is everywhere on earth,…
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Primary Cancer Cells: A Culture System Opens the Door to Individualized Therapy
Self-destruction of the body: Cancer turns normal body cells into their malignant counterpart that wants to keep on replicating by any means. Understanding the individual disease offers the potential for more effective therapies with fewer side effects. The new PromoCell Primary Cancer Culture System allows researchers to generate high-fidelity in vitro models of primary tumors….
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The right choice of endothelial cells for your vascular research
Human umbilical vein cells (HUVECs) are easy to access, so they have been the major source of primary endothelial cells (ECs) for years. But to study adult vascular pathology, bring your endothelial research to the next level – by choosing adult ECs that more accurately resemble in vitro conditions….
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Trouble With Your Primary Cells? Treat Them Nicely!
What seems basic to cell culturing is pure stress for primary cells: thawing, subcultivation and freezing. Even seasoned scientists tend to overlook this aspect, and manipulate their cultures less carefully than they should. Unhealthy, or even dead cells are the sad consequence. Here, you can benefit from an expert’s tips in handling primary cells,…
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Understanding the vasculature with the help of HUVECs
Cells that feed other cells: The endothelium is a layer of cells that line blood vessels, and it is responsible for many tasks, including the transport of essential nutrients. Human umbilical vein endothelial cells (HUVECs) provide an in-vitro model for studying physiological and pathological processes of the vasculature.
“I’ll never forget the first time I added cancer cells to a HUVECs monolayer and watched them interact….
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Why you should know your human mesenchymal stem cells inside out
Doctors, and patients with chronic diseases, are putting hope into new findings in regenerative medicine that are based on mesenchymal stem cell (MSC) research. As promising as some published results sound, they might be not reproducible – simply due to insufficient authentication and poor handling of cell cultures. Rigorous validation of cell identity is crucial for working with human mesenchymal stem cells – and,…
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Macrophages: The high-flyers of biomedical research
One cell type can fight disease, help form blood vessels, regulate cell stability— and more. Macrophages are true masters of multitasking. In our body they are the first line of defense against invading bacteria, fungi and viruses. But they are not only a key element of our immune defense; they can also play a role in destroying tissue,…
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How Oxygen Affects Primary Cell Culture
Oxygen has two faces: While it’s a life-saving gas essential for humans and animals, excessive generation of reactive oxygen species damages cells and promotes senescence. This duality is the research focus of Professor Giovanni E. Mann of King’s College London. Prof. Mann and his colleagues are currently investigating redox signaling in vascular cells in culture….
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Mycoplasma contamination – small organisms cause big trouble
An invisible enemy that reduces the growth rate of your cell lines, mycoplasma threaten your hard work and scientific data. Every fourth cell culture could be affected. But there is hope. Our expert, Dr. Tobi Limke, explains how you can prevent mycoplasma contamination, and she outlines ways to deal with bacteria once they have infiltrated your plates and flasks….
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