Getting started with primary cells – Standard procedures in cell culture
In this quick start guide, we review the finer points of thawing, plating, and passaging primary human cells. Even if you’re experienced using these cells, we review some of the little things that can make a large difference in your cell culture success with our cells. Presented by PromoCell Application Specialist,…
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PromoCell now exclusive supplier of MATra (Magnet-Assisted Transfection)
Primary cells can prove much more difficult to transfect than other commonly used cell lines. While high transfection efficiencies are possible using commercially available transfection reagents in diverse, commonly used cell lines, primary cells but also diverse cell lines have proven much more difficult to transfect….
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Cells in action: Differentiation of human mesenchymal stem cells into neurons
In this video we can see the differentiation of PromoCell umbilical cord mesenchymal stem cells into neurons. Excitingly, to our knowledge this is one of the first high-resolution, long-term, live cell time-lapses showing this process. These cells were grown for 13 days in PromoCell complete mesenchymal stem cell neurogenic differentiation medium prior to capture of this film….
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MSC reproducibility: Towards the standardization of Mesenchymal Stem Cells
For science to move forwards, the research we do must be reproducible. One of the ways we’re helping your work be as consistent as possible is through our characterized MSCs….
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More robust and advanced Pericyte Growth Medium 2 for your lab
– Low-serum formulation for better lot-to-lot consistency
– Guaranteed average population doubling time (PDT) of less than 40 hours for the first 10 population doubles (PDs)
– Former Pericyte Growth Medium (C-28040) available to order for existing customers only until the end of 2019…
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Cells that could lead to new therapies: PromoCell now offers in vitro disease models
– Portfolio extension: Wide range of donor cells with common chronic diseases
– New models can help researchers investigate disease development and find new treatment approaches
– Matched growth media are optimized for pharmaceutical drug discovery…
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Adipocytes: key players in metabolic homeostasis
Adipocytes do more than simply store fat. They also play a role in metabolic diseases. Through better understanding of how adipose cells function and interact, researchers can develop new, targeted therapies for treating adipositas and diabetes.
Our bodies and the bodies of all living organisms work like batteries: we get energy from food,…
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PromoCell announces services for large-scale industrial lots of Mesenchymal Stem Cells
– New services for large-scale industrial lots allows customers to have high volumes of standardized MSC populations derived from bone marrow and adipose tissue
– Guaranteed MSC functionality and phenotype in accordance to ISCT* guidelines
– Optimized media with complete supplementation and expert technical support…
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Cells in Action: Human Primary Keratinocytes at the stratum granulosum
Keratinocytes at the stratum granulosum contain two characteristic structures that are visible in this high resolution footage by Nanolive – the lamellar bodies and keratohyalin granules. While keratohyalin granules contain proteins involved in the aggregation of keratin filaments and in the formation of the cell envelope, lamellar bodies contain lipids and fuse with the plasma membrane in order to secrete the content into the extracellular space….
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Cells in Action: membrane protrusions in HUVECs (Human Umbilical Vein Endothelial Cells)
During angiogenesis, cell-matrix interactions are crucial. The cell matrix structures are involved in migration, invasion and survival of cells throughout the angiogenic process. In this video we can see filopodia and lamellipodia in great detail, thanks to Nanolive imaging’s ability to view membrane deformations. These structures are essential for migration, cell-cell interactions,…
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Cells in Action: Double mitosis of Human Umbilical Vein Endothelial Cells (HUVECs)
Images of cells undergoing mitosis are mind-blowing, and non more beautiful than those of our Human Umbilical Vein Endothelial Cells (HUVECs) captured by Nanolive’s 3D Cell Explorer.
On the right hand side, the cell seems to enter mitosis but the chromatids don’t complete segregation and the cell returns to interphase without dividing….
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Invisible attraction: Using magnets to develop drugs in cell cultures
Modern medicines are high-tech products. Developing medicines increasingly includes high throughput screening by fully automated analysis robots. One exciting high throughput approach uses magnetic 3D cell culture – which provides a more realistic model of human tissue than with 2D cell culture.
The later a candidate drops out, the more expensive it is….
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