RESOURCES
Items 85-96 of 114 Results
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Chondrogenic differentiation and analysis of mesenchymal stem cells (MSC)
Managed in vitro differentiation of mesenchymal stem cells (MSC) into chondrocytes. Multipotent adult stem cells are important for various tissues of the musculoskeletal system.
Application Notes
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Osteogenic differentiation and analysis of mesenchymal stem cells (MSC)
Differentiate mesenchymal stem cells (MSC) into osteoblasts. Read our application note and find out more. The multipotency of these cells is one essential characteristic of MSC, according to the International Society for Cellular Therapy.
Application Notes
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Adipogenic differentiation and analysis of mesenchymal stem cells (MSC)
Turning mesenchymal stem cells (MSC) into adipocytes is the aim of this application note. Research using MSC is a field of high interest and could ultimately lead to new therapies for treating a variety of lifestyle diseases.
Application Notes
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Real-time analysis of stem cell proliferation during differentiation
Here we describe the real-time analysis of human stem cell differentiation using cell-based assays. This allows for quantification of cell viability, proliferation and apoptosis.
Application Notes
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Development of a high throughput 3D in vitro wound healing model
Studying wound healing is a challenge, however the ability to quantifying the difference between 3D skin models is now possible.
Application Notes
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Determination of the Tumorsphere Formation Efficiency (TFE)
Determine the tumorsphere formation efficiency using an assay to quantify the ability of single cells to form spheres. Our defined and xeno-free 3D Tumorsphere Medium enables you to cultivate cancer stem cells.
Application Notes
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Osteoblast differentiation and mineralization
Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. Their differentiation in vitro and in vivo can be characterized in three stages. We describe analysis of different molecular markers of those processes.
Application Notes
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Tumorsphere culture of cancer stem cells
Find out how to cultivate cancer stem cells in tumorspheres using our defined and xeno-free 3D Tumorsphere Medium. This enables you to characterize such cells and study their malignancy.
Application Notes
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Endothelial cell transmigration and invasion assay
Angiogenesis, the development of blood vessels from existing ones, is an essential process. Common in vitro models are the transwell migration assay and the cell invasion assay.
Application Notes
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Mammosphere culture of human breast cancer cells
PromoCell 3D Tumorsphere Medium XF allows robust 3D culture even when using breast-cancer cell lines. Mammospheres are now regarded as highly valuable in vitro models for studies on tumor formation and metastasis.
Application Notes
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Neurosphere culture of brain tumor stem cells
Using 3D cultures of brain cancer cells as neurospheres in serum-free conditions maintains the properties of primary tumors. In vitro models with the 3D Tumorsphere Medium XF are an essential prerequisite for research aimed at identifying ways to eradicate brain tumor cancer stem cells.
Application Notes
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Generation of monocyte-derived Dendritic Cells (moDCs)
We offer a step-by-step protocol for the in vitro maturation of human monocytes into immature monocyte-derived dendritic cells (moDCs), as well as into fully mature moDCs, using PromoCell media.
Application Notes