Items 1-12 of 97 Results
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Epi-Fluorescence Mitochondrial Imaging in Live Single Cells and Tumorspheres
Mitochondria are the organelles that fuel life, which makes them extremely important for research. We offer a special dye for live-cell and fixed-cell imaging. Use this dye to boost your mitochondria research. Find out how.
Application Notes
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Holotomography-Monitored Phagocytosis Assay of M1 Macrophages
Phagocytosis is an essential process for pathogen clearance and is the subject of research for many immunologists. Find out more about PromoCell's phagocytosis assay for fluorescence microscopy and how you can implement this method in your lab.
Application Notes
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Cells in Action: Mitosis in Human Mesenchymal Stem Cells (MSCs)
Watch the process of cell division in PromoCell's human mesenchymal stem cells! These images were captured using Nanolive’s non-invasive technology, which allows us to access the dynamics of biological processes, such as mitosis, with unprecedented resolution.
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Cells in action: Differentiation of human mesenchymal stem cells into neurons
Cells in action: Differentiation of human mesenchymal stem cells into neurons In this video we can see the differentiation of PromoCell umbilical cord mesenchymal stem cells into neurons. Excitingly, to our knowledge this is one of the first high-resolution, long-term, live cell time-lapses showing this process. These cells were grown for 13 days in PromoCell complete mesenchymal stem cell neurogenic differentiation medium prior to capture of this film. Images were captured every 30 seconds over a 20-hour period using NanoLive’s 3D Cell Explorer.
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Endothelial Cell Transmigration and Invasion Assay
Angiogenesis, the development of blood vessels from existing ones, is an essential process. Common in vitro models are the transwell migration assay and the cell invasion assay.
Application Notes
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Tumorsphere Culture of Cancer Stem Cells (CSC) with the PromoCell 3D Tumorsphere Medium XF
Find out how to cultivate cancer stem cells in tumorspheres using our defined and xeno-free 3D Tumorsphere Medium. This enables you to characterize such cells and study their malignancy.
Application Notes
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Extended Neurosphere Culture of Brain Tumor Stem Cells with the PromoCell 3D Tumorsphere Medium XF
Using 3D cultures of brain cancer cells as neurospheres in serum-free conditions maintains the properties of primary tumors. In vitro models with the 3D Tumorsphere Medium XF are an essential prerequisite for research aimed at identifying ways to eradicate brain tumor cancer stem cells.
Application Notes
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3D tumorsphere cell culture model
Explore how 3D cultivation enhances understanding interactions of cancer stem cells and their environment. Discover its versatile applications.
Posters
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Cells in Action: membrane protrusions in HUVECs (Human Umbilical Vein Endothelial Cells)
During angiogenesis, cell-matrix interactions are crucial. The cell matrix structures are involved in migration, invasion and survival of cells throughout the angiogenic process. In this video we can see filopodia and lamellipodia in great detail, thanks to Nanolive imaging's ability to view membrane deformations. These structures are essential for migration, cell-cell interactions, sensing of the cell environment and more. While filopodia appear as long, thin protrusion-like structures that emerge from the cellular membrane, lamellipodia are broad, sheet-shaped structures containing thin and short interconnected actin filaments.
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Cells in Action: Double mitosis of Human Umbilical Vein Endothelial Cells (HUVECs)
Images of cells undergoing mitosis are mind-blowing, and non more beautiful than those of our Human Umbilical Vein Endothelial Cells (HUVECs) captured by Nanolive's 3D Cell Explorer. On the right hand side, the cell seems to enter mitosis but the chromatids don't complete segregation and the cell returns to interphase without dividing. Exiting mitosis is controlled by proteolysis and cyclin dependent kinases (CDKs). Mitosis regulatory machinery sometimes detects errors and forces a return to interphase, as seen here. This kind of research helps develop understanding of the cell cycle, which can be used in myriad applications including cancer research.
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High Throughput 3D Cell Culture
High-throughput 3D cell culture is a technology that has great potential, as it allows more accurate simulation of in vivo conditions. One major drawback is the high cost, which can be overcome by automating certain processes. Find out how.
Brochures
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Cells in Action: Human Primary Keratinocytes at the stratum granulosum
Keratinocytes at the stratum granulosum contain two characteristic structures that are visible in this high resolution footage by Nanolive - the lamellar bodies and keratohyalin granules. While keratohyalin granules contain proteins involved in the aggregation of keratin filaments and in the formation of the cell envelope, lamellar bodies contain lipids and fuse with the plasma membrane in order to secrete the content into the extracellular space.
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