Powered by BiozCancer Cell Line Medium XF
Serum-free and xeno-free medium for the standardized in vitro cultivation of established cancer cell lines. Fibronectin or vitronectin coating required.
Key benefits:
- Suitable for long-term routine culture of adherently growing established human cancer cell lines
- Compatible with most commonly used human cancer cell lines
- Serum-free and xeno-free formulation
- Standardized and reproducible system
Components:
- Cancer Cell Basal Medium
- Cancer Cell SupplementMix
Our Cancer Cell Line Medium XF is the working horse of the cancer cell culture toolbox. It allows you to cultivate or co-cultivate a range of different cancer cells, cancer-associated cells, and non-cancer cells such as immune or stromal cells. This rich growth medium supports the long-term cultivation of cancer cells over many passages.
You can use the Cancer Cell Line Medium XF as a standalone to expand and maintain a cancer cell population in a standardized and reproducible environment. Or you can use it in combination with the Primary Cancer Cell Culture System (PCCS) and the 3D Tumorsphere Medium XF, e.g., to establish cancer cell lines from tumor biopsies.
Due to the flexible nature of our Cancer Cell Line Medium XF, you can use it to support a broad range of cancer cell lines or cancer cells from tumor biopsies while maintaining the tumor-initiating cancer stem cells (CSCs).
Cancer Cell Line Medium XF is serum-free and xeno-free and provides an environment free from non-defined materials, such as fetal calf serum, extracts, or hydrolysates. Cancer Cell Line Medium XF also shows very low lot-to-lot variability.
These properties provide the ideal culture environment to be used for other applications such as co-cultivation of cancer cells and immune cells for cell therapy development like CAR-T cells. The serum- and xeno-free formulation supports the expansion of extracellular vesicle (EV)-producing cancer cells in an EV-free environment.
Note: The serum- and xeno-free formulation means non-availability of attachment factors. Hence, coating with an appropriate adhesion factor is a pre-requisite. For the establishment of the culture conditions, it is recommended to test fibronectin and vitronectin coating.
Cell types tested for serial passage with our Cancer Cell Line Medium XF:
| Tissue | Tested cell lines | Cell lines origin | Remarks |
|---|---|---|---|
| Brain | BV2 | immortalized murine primary microglial cells | coat with fibronectin: 1 µg/cm² |
| Breast | HCC38 | Human breast ductal carcinoma cell line | coat with collagen: 6 µg/cm² |
| Breast | HCC1143 | Human ductal carcinoma cell line | coat with collagen: 6 µg/cm² |
| Breast | HCC1937 | Human ductal carcinoma cell line | coat with collagen: 6 µg/cm² |
| Breast | MCF-7 | pleural effusion of metastatic human breast adenocarcinoma | coat with fibronectin: 1 µg/cm² or with vitronectin: 0.5 µg/cm² |
| Colon | HT-29 | human colon adenocarcinoma | coat with vitronectin: 0.5 µg/cm² |
| Connective tissue | HT1080 | human fibrosarcoma | coat with fibronectin: 1 µg/cm² |
| Liver | HepG2 | hepatocellular carcinoma of the human liver | coat with vitronectin: 0.5 µg/cm² |
| Lung | A-549 | human lung carcinoma | coat with vitronectin: 0.5 µg/cm² |
| Prostate | LNCaP | lymph node metastasis of human prostate adenocarcinoma | 3D culture in C-28070 is recommended |
| Peripheral blood | BDCM | B lymphoblast cell line | no coating required |
| Peripheral blood | HCC38 BL | EBV transformed B lymphoblast cell line | no coating required |
| Peripheral blood | MV-4-11 | Human acute myelogenous leukemia (suspension) | no coating required |
| Bone marrow | KG-1 | Human acute myelogenous leukemia (suspension) | no coating required |
| Kidney | ACHN | Human Renal Cell Carcinoma | coat with fibronectin: 1 µg/cm² |
| Brain | A172 | Human Glioblastoma | coat with fibronectin: 1 µg/cm² |
| Skeletal muscle | C2C12 | Mouse Myoblasts | coat with fibronectin: 1 µg/cm² |
| Skin | B16-F10 | Mouse Melanoma | coat with fibronectin: 1 µg/cm² |
| Abelson murine leukemia virus-induced tumor | RAW264.7 | Mouse Macrophage | coat with fibronectin: 1 µg/cm² |
| Brain, neuroectodermal | Neuro-2a | Mouse Neuroblastoma | coat with fibronectin: 1 µg/cm² |
Recommended products
3D Tumorsphere Medium XF
Serum-free and xeno-free medium for the cultivation of cancer cell lines as 3D tumorspheres.
Freezing Medium Cryo-SFM
Defined, animal-component free, and protein-free cryopreservation medium.