Can I re-freeze PromoCell Normal Human Cells?
Yes, PromoCell Normal Human Cells can be re-frozen after thawing and expansion. However, re-freezing is generally not recommended because each additional freeze-thaw cycle can reduce cell recovery, viability, and proliferation potential.
Please note that after an additional freezing cycle, the number of population doublings (PDs) specified in the Certificate of Analysis (CoA) can no longer be guaranteed.
If re-freezing is required, we recommend freezing the cells at a low passage number and using PromoCell Cryo-SFM Plus (C-29920). Cryo-SFM Plus is a defined, animal component-free, and protein-free cryopreservation medium developed to support optimal storage and recovery of primary human cells.
For best results, avoid unnecessary freeze-thaw cycles and establish working stocks from early-passage cultures whenever possible.
Please note that after an additional freezing cycle, the number of population doublings (PDs) specified in the Certificate of Analysis (CoA) can no longer be guaranteed.
If re-freezing is required, we recommend freezing the cells at a low passage number and using PromoCell Cryo-SFM Plus (C-29920). Cryo-SFM Plus is a defined, animal component-free, and protein-free cryopreservation medium developed to support optimal storage and recovery of primary human cells.
For best results, avoid unnecessary freeze-thaw cycles and establish working stocks from early-passage cultures whenever possible.
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