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I differentiated M1 macrophages from self-sourced PBMCs following your differentiating protocol. In the final cell culture I can see small cells attached to the macrophages which are CD3+ (T-cells). What is the reason for the T-cell contamination after t

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The reason for the higher number of lymphocytes in the macrophage culture is probably due to an insufficient washing step during the purification of the monocyte via adherence. The 3 washing steps in our protocol are essential to receive a monocyte population of over 90%.

I differentiated M1 macrophages from self-sourced PBMCs following your differentiating protocol. In the final cell culture I can see small cells attached to the macrophages which are CD3+ (T-cells). What is the reason for the T-cell contamination after t

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