Following the recommended seeding density for your M1 macrophages (100.000 cells per cm2) I would need too many cells to have a complete 96- or 384-well plate. This is too expensive for me can I reduce the number of cells per cm2?
Description
The recommended seeding density with 100;000 cells per cm2 is needed for a confluent cell layer as the cells do not proliferate.
However; you can reduce the seeding density by the factor 3 to 5 and the macrophages are still viable.