Can I use Growth Medium instead of TNS to inactivate trypsin when splitting cells?
In general, no. To completely inactivate trypsin, a serum concentration of > 10% FBS is required.
Because most PromoCell Growth Media are serum‑reduced or serum‑free, they do not contain sufficient serum to reliably stop trypsin activity. Therefore, the use of a dedicated trypsin inhibitor, such as Trypsin Neutralizing Solution (TNS), is strongly recommended.
Because most PromoCell Growth Media are serum‑reduced or serum‑free, they do not contain sufficient serum to reliably stop trypsin activity. Therefore, the use of a dedicated trypsin inhibitor, such as Trypsin Neutralizing Solution (TNS), is strongly recommended.
Products relevant to this topic
Trypsin Neutralizing Solution - 30 ml
Cat No
C-41100
Trypsin Neutralizing Solution containing 0.05% Trypsin Inhibitor in 0.1% BSA. Available in different sizes.
DetachKit, 3 x 30 ml
Cat No
C-41200
DetachKit for gentle and effective detachment of adherent primary human cells. Contains Trypsin/EDTA (ready-to-use).
Keratinocyte Growth Medium 2 (Ready-to-use)
Cat No
C-20011
Serum-free cell culture medium for keratinocytes from the epidermis.
Keratinocyte Growth Medium 3
Cat No
C-20021
Serum-free and BPE-free cell culture medium for the cultivation of human keratinocytes from the epidermis of juvenile foreskin or adult skin.
Mammary Epithelial Cell Growth Medium (Ready-to-use)
Cat No
C-21010
Serum-free cell culture medium for epithelial cells from the mammary gland.
Airway Epithelial Cell Growth Medium 2
Cat No
C-21040
Serum-free and BPE-free cell culture medium for epithelial cells isolated from large air passages.
Airway Epithelial Cell Growth Medium XF (prf)
Cat No
C-21050
Xeno- and serum-free cell culture medium for epithelial cells isolated from large air passages.
Airway Epithelial Cell Growth Medium (Ready-to-use)
Cat No
C-21060
Ready-to-use serum-freecell culture medium for epithelial cells from large air passages.