Can I defrost Accutase Solution, prepare aliquots and refreeze them?

Yes, Accutase Solution can be defrosted, aliquoted, and then refrozen. Defrosting: Accutase should be defrosted overnight in the refrigerator or placed in a tub of cold tap water. Do not defrost in a 37°C water bath. Stability: Once thawed, it is stable for at least 2 months in the refrigerator if stored promptly after use.