What are the advantages of monocyte enrichment by adherence selection using PromoCell Monocyte Attachment Medium (C‑28051)?
Monocyte enrichment by adherence selection using PromoCell Monocyte Attachment Medium (C‑28051) offers several practical and biological advantages:
Rapid enrichment
Large numbers of adherent monocytes can be obtained within approximately 1.5 hours, making this method very fast compared to bead‑based separation techniques.
High purity
When washing steps are performed correctly, a monocyte purity of 80–90 % can be achieved.
“Untouched” monocytes
This method does not involve magnetic microbeads. Therefore, monocytes are not exposed to bead binding or internalization, resulting in untouched cells with preserved physiological properties.
Improved cellular health
The absence of magnetic beads also prevents phagocytosis of microbeads by monocytes, which can negatively affect cell viability and function.
Time‑saving and cost‑effective
Compared to immunomagnetic separation, adherence‑based enrichment is less labor‑intensive, faster, and more economical, while still delivering reliable results.
Rapid enrichment
Large numbers of adherent monocytes can be obtained within approximately 1.5 hours, making this method very fast compared to bead‑based separation techniques.
High purity
When washing steps are performed correctly, a monocyte purity of 80–90 % can be achieved.
“Untouched” monocytes
This method does not involve magnetic microbeads. Therefore, monocytes are not exposed to bead binding or internalization, resulting in untouched cells with preserved physiological properties.
Improved cellular health
The absence of magnetic beads also prevents phagocytosis of microbeads by monocytes, which can negatively affect cell viability and function.
Time‑saving and cost‑effective
Compared to immunomagnetic separation, adherence‑based enrichment is less labor‑intensive, faster, and more economical, while still delivering reliable results.
