Are endothelial cells α‑SMA‑negative under all circumstances?
No. α‑SMA negativity is not an intrinsic or universal property of endothelial cells.
Scientific findings from multiple research groups, as well as PromoCell’s own internal data, indicate that the presence or absence of α‑SMA is not a reliable marker to define the composition of an endothelial cell population. Endothelial cells can express α‑SMA under certain conditions, and its expression may vary depending on extrinsic factors, such as culture conditions or environmental cues.
For this reason, lot‑specific α‑SMA values are not considered meaningful quality criteria and are not reported in PromoCell Certificates of Analysis (CoAs).
Products relevant to this topic
Human Umbilical Vein Endothelial Cells (HUVEC) single donor
Cat No
C-12200
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of single donor.
Human Umbilical Artery Endothelial Cells (HUAEC)
Cat No
C-12202
Primary Human Umbilical Endothelial Cells isolated from the arteries of the umbilical cord.
Human Umbilical Vein Endothelial Cells (HUVEC) pooled
Cat No
C-12203
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of pooled donors.
Human Umbilical Vein Endothelial Cells (HUVEC) pre-screened
Cat No
C-12205
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of pre-screened donors.
Human Umbilical Vein Endothelial Cells (HUVEC) isolated in Growth Medium 2, single donor
Cat No
C-12206
HUVEC isolated from the umbilical vein of single, pooled or pre-screened donors in Endothelial Cell Growth Medium 2 containing additional VEGF.
Human Umbilical Vein Endothelial Cells (HUVEC) isolated in Growth Medium 2, pooled
Cat No
C-12208
HUVEC isolated from the umbilical vein of single, pooled or pre-screened donors in Endothelial Cell Growth Medium 2 containing additional VEGF.
Human Dermal Microvascular Endothelial Cells (HDMEC) juvenile foreskin
Cat No
C-12210
Primary Human Dermal Microvascular Endothelial Cells isolated from the dermis of juvenile foreskin and adult skin.
Human Dermal Blood Endothelial Cells (HDBEC) juvenile foreskin
Cat No
C-12211
Primary Human Dermal Blood Endothelial Cells isolated from different sources (e.g. juvenile foreskin). CD31 and Dil-Ac-LDL uptake positive, Podoplanin negative.