Should skeletal muscle cells be split after adding Skeletal Muscle Cell Differentiation Medium (C‑23061)?
No. Skeletal muscle cells should not be split after the differentiation medium has been added.
Once PromoCell Skeletal Muscle Cell Differentiation Medium (C‑23061) is applied, myoblasts initiate differentiation into myotubes and stop proliferating. At this stage, further passaging or splitting is no longer appropriate.
Recommended workflow
Differentiated myotubes can be difficult to trypsinize. If detachment is necessary for your application, a rubber policeman can be used to gently remove the cells.
Once PromoCell Skeletal Muscle Cell Differentiation Medium (C‑23061) is applied, myoblasts initiate differentiation into myotubes and stop proliferating. At this stage, further passaging or splitting is no longer appropriate.
Recommended workflow
- Before differentiation: Plate skeletal muscle cells directly into the final culture vessels required for your experiment (e.g. multiwell plates).
- After differentiation induction: Perform experiments directly on differentiated myotubes, without further subculturing.
Differentiated myotubes can be difficult to trypsinize. If detachment is necessary for your application, a rubber policeman can be used to gently remove the cells.
Products relevant to this topic
Human Skeletal Muscle Cells (SkMC)
Cat No
C-12530
Primary Human Skeletal Muscle Cells isolated from different skeletal muscles from adult single donors.
Skeletal Muscle Cell Growth Medium (Ready-to-use)
Cat No
C-23060
Ready-to-use low-serum cell culture medium for growth of skeletal muscle cells.
Skeletal Muscle Cell Differentiation Medium (Ready-to-use)
Cat No
C-23061
Defined and animal-component free cell culture medium for differentiation of skeletal muscle cells.