Technical library

Yes. Accutase can be used to detach PromoCell Normal Human Cells.
Accutase acts very gently on cells and does not damage cell membranes or surface epitopes. It is therefore particularly suitable for applications that require unchanged surface markers, such as flow cytometry, or for the detachment of very sensitive cell types.

For best results, we recommend using HEPES BSS to wash cells prior to trypsinization.
PromoCell Trypsin/EDTA (ready‑to‑use) and PromoCell TNS (Trypsin Neutralizing Solution) are both based on HEPES‑buffered Salt Solution (HEPES BSS). Using HEPES BSS for the washing step ensures optimal compatibility with these reagents.
Phosphate‑buffered saline (PBS) can also be used for washing; however, HEPES BSS is normally the preferred option.

We recommend using the PromoCell DetachKit (C‑41200) for subculturing PromoCell normal human cells.
The DetachKit was specifically designed for the safe and efficient detachment of primary human cells during routine subculture and consists of three ready‑to‑use components:

• HEPES BSS (HEPES‑buffered Saline Solution)
• Trypsin/EDTA Solution
• TNS (Trypsin Neutralizing Solution)

Important note
Many PromoCell culture media contain low serum levels or no serum at all. These media are not suitable for inactivating trypsin during subculture.
The Trypsin Neutralizing Solution included in the DetachKit ensures reliable and controlled trypsin inactivation under serum‑free or low‑serum conditions.

Alternative detachment method
As an alternative, PromoCell Accutase Solution may be used for certain cell types.
Please refer to the Manual of the respective cell type for guidance on whether Accutase is suitable and recommended.

The population doubling time (PDT; also called generation time, tg) is typically calculated during the logarithmic (exponential) growth phase of a cell culture. It describes the time required for a cell population to double in number.
 
Formula 
tg = t / n
 
where:
- tg = population doubling time (hours)
- t = time interval (in hours) between two cell counts
- n = number of population doublings during this time interval

Certificates of Analysis (CoA) for PromoCell cells can be downloaded directly from the PromoCell CoA search page.

How to retrieve your CoA

• Go to the PromoCell CoA search page.
• Enter the lot number shown on the cryovial or tissue culture flask.
• Press Enter or click the Search icon to retrieve the corresponding Certificate of Analysis.

 

To culture PromoCell Normal Human Cells, Adult Stem Cells, and Blood Cells, you should have basic experience working under sterile conditions, including routine work under a laminar flow hood.
Experience with other cell types and/or established cell lines is advantageous, as it helps with understanding general cell culture techniques and best practices.
If you are new to cell culture or are in the process of setting up a cell culture laboratory, PromoCell provides scientific support and guidance to help you successfully establish and work with PromoCell Normal Human Cells.

Serum turbidity is most commonly caused by cryoprecipitation of lipid components that occurs during freezing and thawing.
The more frequently serum is subjected to freeze–thaw cycles, the more pronounced the turbidity typically becomes. This effect does not necessarily indicate contamination or loss of functionality, but rather physical precipitation.
 
How to minimize serum turbidity
To reduce turbidity, we recommend the following handling practices:

• Aliquot the serum before freezing
• Store aliquots at −20°C
• Thaw only the required aliquot immediately before use
• Avoid repeated freeze–thaw cycles

The differentiation of human mesenchymal stem cells (hMSCs) into mature adipocytes takes approximately 2 weeks.

• After differentiation, the adipocytes can be maintained for up to 3 weeks in MSC Adipogenic Differentiation Medium.
• For longer‑term culture beyond 3 weeks, we recommend switching to PromoCell Adipocyte Nutrition Medium (C‑27438), which is specifically designed to support the maintenance of mature adipocytes.

When neuronally differentiated MSCs are cultured with regular medium changes every 2-3 days, they can typically be maintained in culture for up to 2 weeks.

Human MSCs are a heterogeneous population, and not all cells will differentiate into osteoblasts.
Differentiation efficiency depends strongly on the tissue source:

• hMSC‑BM: very good osteogenic differentiation
• hMSC‑AT: very good osteogenic and adipogenic differentiation
• hMSC‑UC: weak osteogenic differentiation, mainly chondrogenic

To obtain a high percentage of osteoblast‑like cells, hMSC‑BM or hMSC‑AT are the preferred sources. Please note that lot‑to‑lot variability exists, and differentiation capacity decreases with increasing passage number.
If particularly strong osteogenic differentiation is required, PromoCell Scientific Support can assist with lot selection prior to ordering.