Powered by BiozAirway Epithelial Cell Growth Medium 2
Serum- and BPE-free cell culture medium for epithelial cells isolated from large air passages.
Unit
500 ml
Cat.No.
C-21040
Ready to ship
Our Airway Epithelial Cell Growth Medium 2 is a serum- and BPE-free formulation optimized for the in vitro cultivation of epithelial cells from large air passages, including HBEpC, HNEpC, and HTEpC.
Key features
- Optimized for in vitro cultivation of epithelial cells from large air passages (HBEpC, HNEpC, HTEpC)
- Serum-free and BPE-free formulation
- Supplied as a 500 ml bottle of Airway Epithelial Cell Basal Medium 2 with premixed SupplementMix
- Contains phenol red
- Average population doubling time (including lag phase) over 3 passages ≤70 h
Product benefits
- Physiologically relevant growth: Supports cell growth, cobblestone morphology, and functionality that closely mimics the natural in vivo airway environment without the need for small-molecule additives.
- Enhanced proliferation: Promotes higher population doubling across all airway epithelial cell types compared to our standard growth medium.
- Complete BPE-free ALI culture system: Enables isolation, cultivation, and differentiation of bronchial epithelial cells entirely in a serum- and BPE-free environment when used with our Air–Liquid Interface Medium.
- Improved 3D culture integrity: Maintains high and stable TEER values (≥500 Ω·cm²) for at least 14 days.
- Supports primary cell isolation: Proven performance for isolating HBEpC, HNEpC, and HTEpC from fresh bronchial, nasal, and tracheal tissues without additional medium components.
- Functional airway organoids: Supports the formation of rotating spheroid-like structures with predominantly apical-out cilia for ≥28 days when embedded in basal membrane extract.
Figure 1.Typical small and homogenous cobblestone morphology of HBEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Figure 2.Maintenance of typical cobblestone morphology in highly proliferative HNEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Figure 3.HTEpC isolated and expanded in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Figure 4.Increased cell yield of isolated HNEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Figure 5.Growth curve of primary human HBEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Figure 6.Growth curve of primary human HTEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Figure 7.Growth curve of primary human HNEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Figure 8.Cell identity of isolated HTEpC in serum- and BPE-free Airway Epithelial Cell Growth Medium 2.
Recommended products
Air-Liquid Interface Medium
Serum-free and BPE-free cell culture medium for an optimal and standardized culture of human bronchial epithelial cells at the air-liquid interface (ALI).
Human Bronchial Epithelial Cells (HBEpC)
Primary Human Bronchial Epithelial Cells isolated from the surface epithelium of human bronchi.
Human Nasal Epithelial Cells (HNEpC)
Primary Human Nasal Epithelial Cells isolated from normal human nasal mucosa.
Human Tracheal Epithelial Cells (HTEpC)
Primary Human Tracheal Epithelial Cells isolated from surface epithelium of human trachea.
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Defined, animal-component free, and protein-free cryopreservation medium for optimal cell storage.