Why is Human Serum Albumin (HSA) added to the PBS wash after macrophage detachment?
The Macrophage Detachment Solution (C‑41330) acts directly on the cell membrane to enable efficient detachment of macrophages.
Following detachment, the cells are in a particularly sensitive state. The addition of Human Serum Albumin (HSA) to the PBS wash buffer serves an important protective function:
Following detachment, the cells are in a particularly sensitive state. The addition of Human Serum Albumin (HSA) to the PBS wash buffer serves an important protective function:
- HSA supports regeneration and stabilization of the cell membrane
- It protects macrophages from detrimental effects during the critical phase immediately after detachment
Products relevant to this topic
Macrophage Detachment Solution
Cat No
C-41330
Chemically defined, non-enzymatic detachment solution optimized for gentle detachment of strongly adherent macrophages.
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12914
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M1-Macrophage Generation Medium XF (incl. GM-CSF).
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12915
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M2-Macrophage Generation Medium XF (incl. M-CSF).