What viability can be expected after detaching macrophages with Macrophage Detachment Solution?

The viability of detached macrophages depends largely on whether the cells can be harvested using Macrophage Detachment Solution alone or whether additional mechanical detachment is required.

• If the macrophages can be detached using Macrophage Detachment Solution alone (i.e. without scraping), the expected viability is typically 50-80%.
• If a cell scraper must be used in addition to the Macrophage Detachment Solution, viability is usually below 50% and may be as low as ~30%.

Please note: Macrophages are highly adherent cells, and some loss of viability during detachment is expected. To maximize recovery and viability, mechanical stress should be minimized whenever possible.