Technical library

The recommended media for a particular cell type are specified on the respective product page ("Recommended Products") and can be found in the Manual belonging to the cells.

The recommended Growth Media are specified in the Manual belonging to the cells and can be also found on the product page of the cells ("Recommender products").

Our standard protocol recommends performing trypsinization at room temperature (RT).and to monitor cell detachment under the microscope. Prolonged trypsinization at 37°C can lead to irreversible damage of the cells.

However, in some cases, we recommend trypsinization at 37°C. The manual specifies the duration of trypsinization in these cases.

Yes, RNAlater Solution will denature proteins. Therefore, protein obtained from PromoCell cell pellets will be suitable for applications such as Western blotting or 2D gel electrophoresis, but not for applications that require native protein.

PromoCell cell pellets (C-14**) are an easily accessible source of DNA, RNA, and proteins.

No, the PromoCell cell pellets (C-14**) are frozen at -20°C and cannot be revived. Their main application is to analyze RNA or protein. Cryopreserved cells that can be revived are available from the same donors. Please contact our Technical Customer Support if you need matched viable cells.

Slow growth after subculture can be caused by over-trypsinization or other suboptimal culture conditions. Please see attached trouble shooting guide for possible reasons.

Poor attachment after thawing can be a result of inappropriate freezing, storing or thawing the cells as well as from inadequate culture conditions (medium, incubator). The attached trouble shooting guide should help you to identify the possible reasons.

• DC Base Medium (C-28053) consists of 250 ml Basal Medium + SupplementMix. It does not include cytokines and must therefore be supplemented according to the user's individual needs.

• DC Generation Medium (C-28050) is ready-to-use and consists of 250 ml Basal Medium + SupplementMix + cytokines.

The Monocyte Attachment Medium allows for the efficient adherence selection (within 1 hr) of monocytes from freshly isolated mononuclear cells while maintaining optimal cell health. The Time-consuming and costly immunomagnetic purification of monocytes prior to DC generation is not necessary and can be skipped when using this medium.