Technical library

The Cytokine Mix M1 and M2 should not be subjected to further freeze/thaw cycles.

You could probably use our MSC Chondrogenic Differentiation Medium without inducers (C-28014). It is also serum-free and the same as C-28012 just without chondrogenic inducers.

We strongly advise against overnight incubation. The Monocyte Attachment Medium does not contain cytokines/survival factors for the monocytes. If the cells remain in the Monocyte Attachment Medium for longer than 2 hours; they will go into apoptosis and die. The lymphocytes will survive longer. Some of them will attach after such a long time and then cannot be washed off. Therefore; overnight attachment is absolutely inappropriate. However; if time is short; monocyte attachment can be shortened to 1 hour. To do this; it is best to add Monocyte Attachment Medium to the culture vessels in advance and equilibrate it in the incubator. Remove the vessels from the incubator only briefly to add the appropriate amount of concentrated PBMC suspension. Then; even after 1 hour; most of the monocytes are attached.

We have never tested the cultivation of our assay-ready macrophages in MEM alpha + FBS. We cannot predict whether it will work and therefore strongly recommend the use of our M1 Macrophage Generation Medium XF and fibronectin-coated vessels.

Our cells are not manufactured according to GMP guidelines and are intended for in vitro use only. Our EXCiPACT™ GMP certification only applies to the processes related to the media.