I would like to know how homogenous the MSC population is and if all cells become osteoblasts when grown in MSC Osteogenic Differentiation Medium (C-28013).

Human Mesenchymal Stem Cells have retained the ability to differentiate into a variety of cell types including fat cells, chondrocytes, and osteoblasts. PromoCell supply hMSC from 3 different tissues: bone marrow (hMSC-BM; C-12974), umbilical cord matrix (hMSC-UC; C-12971), and adipose tissue (hMSC-AT; C-12977). MSC-BM show very good differentiation into bone cells but also into chondrocytes and fat cells when the respective Differentiation Media is used. MSC-UC have a high potential to differentiate into chondrocytes but only weak potential for fat or bone cell differentiation. In contrast, MSC-AT differentiate very well into fat and bone cells but only moderately into chondrocytes. In other words, to obtain a high percentage of bone cells, MSC-BM or MSC-AT are the cells of choice. There are of course lot-to-lot variations and the differentiation will decrease from passage to passage. If you need MSCs with a particularly high osteoblast differentiation capacity, you can contact our Scientific Support before placing your order so that we can select an appropriate cell lot for you.

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Q: How can I calculate the population doubling time?

The population doubling time or generation time (t g ) is usually calculated during the logarithmic phase of growth. It specifies the time (t) in hours needed by the culture to double its cell number. t g = t / n t = time (hrs) between 2 cell counts n= number of population doublings

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Upon arrival, the basal media should be stored between 4°C and 8°C, the supplements at -20°C.

Please do not freeze our cell culture media. Freezing can lead to irreversible precipitation of media components and the quality can no longer be assured.

Yes, you can use accutase to detach Normal Human Cells. Accutase acts very gently on the cells. Cell membranes and surface epitopes will not be harmed. It is therefore mostly used for applications that require unchanged surface markers, e.g. for flow cytometry, or for detachment of very sensitive cells.

The PromoCell Trypsin/EDTA (ready-to-use) and the PromoCell TNS solution are both based on HepesBSS. Therefore, it is best to use HepesBSS to wash the cells prior to trypsinization.
However, PBS can also be used.

We recommend using the DetachKit (C-41200) for subculturing our normal human cells.

It was designed for the safe and efficient detachment of primary human cells in routine subculturing and consists of 3 components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralizing Solution). All 3 components are ready-to-use.

Please note: Many of our culture media have low serum content or no serum at all. These media are not suitable for inactivating trypsin during subculture.

The population doubling time or generation time (t_g) is usually calculated during the logarithmic phase of growth. It specifies the time (t) in hours needed by the culture to double its cell number.

t_g = t / n

t = time (hrs) between 2 cell counts

n= number of population doublings

The Certificates of Analysis can easily be downloaded from our CoA search page. Simply type in the lot number indicated on the cryovial/TC-flask and click the SEARCH button.

You need to have experience working under sterile conditions and under a laminar flow hood. It is of advantage to have experience with other cell types and/or cell lines. If you are a beginner in cell culture and would like to establish a cell culture lab, we will assist you in working with PromoCell Normal Human Cells.

Serum turbidity is usually caused by cryoprecipitation of lipid components during freezing and thawing. The more times the serum is subjected to freeze/thaw cycles, the more turbidity is noticed.

It can be minimized by freezing the serum in aliquots at -20°C and thawing these aliquots individually at the time of use.

Differentiation of hMSC into mature adipocytes takes approx. 2 weeks. You can keep the adipocytes for up to 3 weeks in the MSC Adipogenic Differentiation Medium. After 3 weeks we recommend to switch to PromoCell's Adipocyte Nutrition Medium (C-27438).

Changing the medium every 2-3 days, the neuronally differentiated MSC can be kept in culture for up to 2 weeks.

Human Mesenchymal Stem Cells have retained the ability to differentiate into a variety of cell types including fat cells, chondrocytes, and osteoblasts. PromoCell supply hMSC from 3 different tissues: bone marrow (hMSC-BM; C-12974), umbilical cord matrix (hMSC-UC; C-12971), and adipose tissue (hMSC-AT; C-12977).

MSC-BM show very good differentiation into bone cells but also into chondrocytes and fat cells when the respective Differentiation Media is used.
MSC-UC have a high potential to differentiate into chondrocytes but only weak potential for fat or bone cell differentiation.
In contrast, MSC-AT differentiate very well into fat and bone cells but only moderately into chondrocytes.

In other words, to obtain a high percentage of bone cells, MSC-BM or MSC-AT are the cells of choice. There are of course lot-to-lot variations and the differentiation will decrease from passage to passage. If you need MSCs with a particularly high osteoblast differentiation capacity, you can contact our Scientific Support before placing your order so that we can select an appropriate cell lot for you.