How long should cryopreserved macrophages be activated to measure cytokine release?
In general, cryopreserved macrophages should be activated for approximately 24 hours, or at least overnight, to reliably measure cytokine release.
If cytokine induction is not optimal under your specific experimental conditions, the activation time can be shortened or extended accordingly to optimize the response.
If cytokine induction is not optimal under your specific experimental conditions, the activation time can be shortened or extended accordingly to optimize the response.
Products relevant to this topic
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12914
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M1-Macrophage Generation Medium XF (incl. GM-CSF).
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12915
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M2-Macrophage Generation Medium XF (incl. M-CSF).
M1-Macrophage Generation Medium XF
Cat No
C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
M2-Macrophage Generation Medium XF
Cat No
C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.