Can I shorten the centrifugation time to 10 minutes at 350×g after detaching macrophages for flow cytometry?
No. We do not recommend shortening the centrifugation time.
Centrifugation at 350×g for 15 minutes has been validated and tested by PromoCell and is also used during quality control testing.
Reducing the centrifugation time or g‑force may result in significant cell loss, as intact macrophages may not sediment completely.
Recommendation
To ensure reliable recovery of macrophages for flow cytometric analysis, please follow the validated centrifugation conditions of 15 minutes at 350×g.
Centrifugation at 350×g for 15 minutes has been validated and tested by PromoCell and is also used during quality control testing.
Reducing the centrifugation time or g‑force may result in significant cell loss, as intact macrophages may not sediment completely.
Recommendation
To ensure reliable recovery of macrophages for flow cytometric analysis, please follow the validated centrifugation conditions of 15 minutes at 350×g.
Products relevant to this topic
M1-Macrophage Generation Medium XF
Cat No
C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
M2-Macrophage Generation Medium XF
Cat No
C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.
Macrophage Detachment Solution
Cat No
C-41330
Chemically defined, non-enzymatic detachment solution optimized for gentle detachment of strongly adherent macrophages.