Can I trypsinize PromoCell normal human cells at 37°C?
The standard PromoCell protocol recommends performing trypsinization at room temperature (RT) while monitoring cell detachment under the microscope.
Prolonged trypsinization at 37°C may lead to irreversible cell damage and reduced cell viability.
Exceptions
For certain cell types, trypsinization at 37°C may be recommended.
In these cases, the Instruction Manual of the respective cell type specifies the temperature and exact duration of trypsinization.
Prolonged trypsinization at 37°C may lead to irreversible cell damage and reduced cell viability.
Exceptions
For certain cell types, trypsinization at 37°C may be recommended.
In these cases, the Instruction Manual of the respective cell type specifies the temperature and exact duration of trypsinization.
Products relevant to this topic
Trypsin/EDTA (ready-to-use), 30 ml
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Trypsin/EDTA (ready-to-use) solution. Available in different sizes.
DetachKit, 3 x 30 ml
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DetachKit for gentle and effective detachment of adherent primary human cells. Contains Trypsin/EDTA (ready-to-use).
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, pooled
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskin isolated in Growth Medium 3, single donor
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, single donor
Cat No
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.