Use this protocol to generate cancer organoids easily and reliably. This uses normal human primary dermal fibroblasts (NHDFs) co-cultured with MCF-7 cells but also serves as a flexible template for other cell types. Using Cancer Cell Line Medium XF, it also eliminates undefined components, such as fetal calf serum, extracts, or hydrolysates.
![](https://promocell.com/wp-content/uploads/2022/05/App-Note-Cancer-Organoid-724x1024.png)