Generation of a 3D human airway model with primary human nasal and tracheal epithelial cells using our Air-Liquid Interface Medium (ALI-Airway).
This straightforward protocol will walk you through the initial 2D phases of culture and expansion of primary human nasal and tracheal epithelial cells. You’ll then move to the 3D phases of proliferation and differentiation using our Air-Liquid Interface (ALI) Culture System.
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