Do you have recommendations for plasticware for monocyte enrichment and macrophage differentiation?
In general, PromoCell does not recommend a specific plasticware.
However, for macrophage differentiation, the choice of plasticware can significantly influence experimental outcomes.
PromoCell experience
When using PromoCell Dendritic Cell and Macrophage Generation Media, we have observed that the surface properties of the cultureware can strongly affect:
For M1-/M2-Macrophage Generation Media XF, PromoCell recommends Nuncâ„¢ plasticware with a Nunclonâ„¢ surface, as this surface provides more consistent results with respect to cell attachment, differentiation performance, and detachment behavior.
However, for macrophage differentiation, the choice of plasticware can significantly influence experimental outcomes.
PromoCell experience
When using PromoCell Dendritic Cell and Macrophage Generation Media, we have observed that the surface properties of the cultureware can strongly affect:
- Detachment efficiency (differences of up to ~20%)
- Differentiation efficiency itself
For M1-/M2-Macrophage Generation Media XF, PromoCell recommends Nuncâ„¢ plasticware with a Nunclonâ„¢ surface, as this surface provides more consistent results with respect to cell attachment, differentiation performance, and detachment behavior.
Products relevant to this topic
Monocyte Attachment Medium
Cat No
C-28051
Defined, animal-component free, and protein-free medium for efficient adherence selection of monocytes from freshly isolated human mononuclear cells.
M1-Macrophage Generation Medium XF
Cat No
C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
M2-Macrophage Generation Medium XF
Cat No
C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.
Macrophage Base Medium XF
Cat No
C-28057
User-customizable, serum-free and xeno-free medium for generation of macrophages from fresh monocytes. Medium does not include cytokines.